abstract：:In fibroblasts, platelet-derived growth factor receptor alpha (PDGFRα) is upregulated during growth arrest and compartmentalized to the primary cilium. PDGF-AA mediated activation of the dimerized ciliary receptor produces a phosphorylation cascade through the PI3K-AKT and MEK1/2-ERK1/2 pathways leading to the activat...

journal_title：Journal of cell science

authors： Clement DL,Mally S,Stock C,Lethan M,Satir P,Schwab A,Pedersen SF,Christensen ST

更新日期：2013-02-15 00:00:00

abstract：:Mitochondrial DNA (mtDNA) replication and transcription are crucial for cell function, but these processes are poorly understood at the single-cell level. We describe a novel fluorescence in situ hybridization protocol, called mTRIP (mitochondrial transcription and replication imaging protocol), that reveals simultane...

journal_title：Journal of cell science

authors： Chatre L,Ricchetti M

更新日期：2013-02-15 00:00:00

abstract：:Dyneins are motor proteins that move along microtubules. They have many roles in the cell. They drive the beating of cilia and flagella, move cargos in the cytoplasm and function in the mitotic spindle. Dyneins are large and complex protein machines. Until recently, the way they move was poorly understood. In 2012, tw...

journal_title：Journal of cell science

authors： Carter AP

更新日期：2013-02-01 00:00:00

abstract：:Mesenchymal stromal progenitor cells (MSCs) are multipotent progenitors that can be isolated from numerous tissues. MSCs can undergo osteogenic differentiation under proper stimuli. We have recently demonstrated that bone morphogenetic protein 9 (BMP9) is one of the most osteogenic BMPs. As one of the least studied BM...

journal_title：Journal of cell science

authors： Hu N,Jiang D,Huang E,Liu X,Li R,Liang X,Kim SH,Chen X,Gao JL,Zhang H,Zhang W,Kong YH,Zhang J,Wang J,Shui W,Luo X,Liu B,Cui J,Rogers MR,Shen J,Zhao C,Wang N,Wu N,Luu HH,Haydon RC,He TC,Huang W

更新日期：2013-01-15 00:00:00

abstract：:During muscle development myosin molecules form symmetrical thick filaments, which integrate with the thin filaments to produce the regular sarcomeric lattice. In Drosophila indirect flight muscles (IFMs) the details of this process can be studied using genetic approaches. The weeP26 transgenic line has a GFP-encoding...

journal_title：Journal of cell science

authors： Orfanos Z,Sparrow JC

更新日期：2013-01-01 00:00:00

abstract：:During development, general body plan information must be translated into distinct morphologies of individual cells. Shaping cells is thought to involve cortical cytoskeletal components and Bin-Amphiphysin-Rvs167 (BAR) superfamily proteins. We therefore conducted comprehensive side-by-side loss-of-function studies of ...

journal_title：Journal of cell science

authors： Schüler S,Hauptmann J,Perner B,Kessels MM,Englert C,Qualmann B

更新日期：2013-01-01 00:00:00

abstract：:The constant shedding and renewal of epithelial cells maintain the protection of epithelial barriers. Interference with the processes of host cell-cycle regulation and barrier integrity permits the bacterial pathogen Neisseria gonorrhoeae to effectively colonize and invade epithelial cells. Here, we show that a gonoco...

journal_title：Journal of cell science

authors： Vielfort K,Söderholm N,Weyler L,Vare D,Löfmark S,Aro H

更新日期：2013-01-01 00:00:00

abstract：:In plants, many nucleus-encoded proteins are targeted to both mitochondria and plastids, and this process is generally mediated by ambiguous N-terminal targeting sequences that are recognized by receptors on both organelles. In many algae, however, plastids were acquired by secondarily engulfing green or red algae, wh...

journal_title：Journal of cell science

authors： Hirakawa Y,Burki F,Keeling PJ

更新日期：2012-12-15 00:00:00

abstract：:The repair of injured tissue must occur rapidly to prevent microbial invasion and maintain tissue integrity. Epithelial tissues in particular, which serve as a barrier against the external environment, must repair efficiently in order to restore their primary function. Here we analyze the effect of different parameter...

journal_title：Journal of cell science

authors： Abreu-Blanco MT,Verboon JM,Liu R,Watts JJ,Parkhurst SM

更新日期：2012-12-15 00:00:00

abstract：:How cell fate becomes restricted during somatic cell differentiation is a long-lasting question in biology. Epigenetic mechanisms not present in pluripotent cells and acquired during embryonic development are expected to stabilize the differentiated state of somatic cells and thereby restrict their ability to convert ...

journal_title：Journal of cell science

authors： Pasque V,Radzisheuskaya A,Gillich A,Halley-Stott RP,Panamarova M,Zernicka-Goetz M,Surani MA,Silva JC

更新日期：2012-12-15 00:00:00

abstract：:Anionic phospholipids PI(4,5)P2 and phosphatidylserine (PS) are enriched in the cytosolic leaflet of the plasma membrane where endocytic sites form. In this study, we investigated the roles of PI(4,5)P2 and PS in clathrin-mediated endocytosis (CME) site initiation and vesicle formation in Saccharomyces cerevisiae. Liv...

journal_title：Journal of cell science

authors： Sun Y,Drubin DG

更新日期：2012-12-15 00:00:00

abstract：:BMP-dependent patterning in the Drosophila melanogaster wing imaginal disc serves as a paradigm to understand how morphogens specify cell fates. The observed profile of the transcriptional response to the graded signal of BMP relies upon two counter-active gradients of pMad and Brinker (Brk). This patterning model is ...

journal_title：Journal of cell science

authors： Ziv O,Finkelstein R,Suissa Y,Dinur T,Deshpande G,Gerlitz O

更新日期：2012-12-01 00:00:00

abstract：:Cell chemotaxis, such as migration of fibroblasts towards growth factors during development and wound healing, requires precise spatial coordination of signalling events. Phosphoinositides and signalling enzymes involved in their generation and hydrolysis have been implicated in regulation of chemotaxis; however, the ...

journal_title：Journal of cell science

authors： Martins M,Warren S,Kimberley C,Margineanu A,Peschard P,McCarthy A,Yeo M,Marshall CJ,Dunsby C,French PM,Katan M

更新日期：2012-12-01 00:00:00

abstract：:Cyclin G (CycG) belongs to the atypical cyclins, which have diverse cellular functions. The two mammalian CycG genes, CycG1 and CycG2, regulate the cell cycle in response to cell stress. Detailed analyses of the role of the single Drosophila cycG gene have been hampered by the lack of a mutant. We generated a null mut...

journal_title：Journal of cell science

authors： Nagel AC,Fischer P,Szawinski J,La Rosa MK,Preiss A

更新日期：2012-11-15 00:00:00

abstract：:Inositol-(1,4,5)-triphosphate receptors (InsP(3)Rs) are ligand-gated Ca(2+) channels that control Ca(2+) release from intracellular stores and play a central role in a wide range of cellular responses. In most epithelial cells, InsP(3)Rs are not uniformly distributed within the endoplasmic reticulum (ER) membrane, wit...

journal_title：Journal of cell science

authors： Dingli F,Parys JB,Loew D,Saule S,Mery L

更新日期：2012-11-15 00:00:00

abstract：:Increases in cytosolic Ca(2+) concentration ([Ca(2+)](c)) mediated by inositol (1,4,5)-trisphosphate [Ins(1,4,5)P(3), hereafter InsP(3)] regulate activities that include division, contraction and cell death. InsP(3)-evoked Ca(2+) release often begins at a single site, then regeneratively propagates through the cell as...

journal_title：Journal of cell science

authors： Olson ML,Sandison ME,Chalmers S,McCarron JG

更新日期：2012-11-15 00:00:00

abstract：:B chromosomes are centric chromosomal fragments present in thousands of eukaryotic genomes. Because most B chromosomes are non-essential, they can be lost without consequence. In order to persist, however, some B chromosomes can impose strong forms of intra-genomic conflict. An extreme case is the paternal sex ratio (...

journal_title：Journal of cell science

authors： Swim MM,Kaeding KE,Ferree PM

更新日期：2012-11-01 00:00:00

abstract：:A key signaling event that is responsible for gradient sensing in eukaryotic cell chemotaxis is a phosphatidylinositol (PtdIns) lipid reaction system. The self-organization activity of this PtdIns lipid system induces an inherent polarity, even in the absence of an external chemoattractant gradient, by producing a loc...

journal_title：Journal of cell science

authors： Shibata T,Nishikawa M,Matsuoka S,Ueda M

更新日期：2012-11-01 00:00:00

abstract：:Rab GTPases regulate various membrane trafficking pathways but the mechanisms by which GTPase-activating proteins recognise specific Rabs are not clear. Rab8 is involved in controlling several trafficking processes, including the trafficking of transferrin receptor from the early endosome to the recycling endosome. He...

journal_title：Journal of cell science

authors： Vaibhava V,Nagabhushana A,Chalasani ML,Sudhakar C,Kumari A,Swarup G

更新日期：2012-11-01 00:00:00

abstract：:Phosphorylation regulates assembly and disassembly of proteins during endocytosis. In yeast, Prk1 and Ark1 phosphorylate factors after vesicle internalization leading to coat disassembly. Scd5, a protein phosphatase-1 (PP1)-targeting subunit, is proposed to regulate dephosphorylation of Prk1/Ark1 substrates to promote...

journal_title：Journal of cell science

authors： Chi RJ,Torres OT,Segarra VA,Lansley T,Chang JS,Newpher TM,Lemmon SK

更新日期：2012-10-15 00:00:00

abstract：:Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) triggers apoptosis selectively in tumor cells through interaction with TRAIL-R1/DR4 or TRAIL-R2/DR5 and this process is considered a promising avenue for cancer treatment. TRAIL resistance, however, is frequently encountered and hampers anti-cancer ...

journal_title：Journal of cell science

authors： Azijli K,Yuvaraj S,Peppelenbosch MP,Würdinger T,Dekker H,Joore J,van Dijk E,Quax WJ,Peters GJ,de Jong S,Kruyt FA

更新日期：2012-10-01 00:00:00

abstract：:The function of microtubules depends on their arrangement into highly ordered arrays. Spatio-temporal control over the formation of new microtubules and regulation of their properties are central to the organization of these arrays. The nucleation of new microtubules requires γ-tubulin, an essential protein that assem...

journal_title：Journal of cell science

authors： Teixidó-Travesa N,Roig J,Lüders J

更新日期：2012-10-01 00:00:00

abstract：:The morphogenesis-related NDR kinase (MOR) pathway regulates morphogenesis in fungi. In spite of the high conservation of its components, impairing their functions results in highly divergent cellular responses depending on the fungal species. The reasons for such differences are unclear. Here we propose that the spec...

journal_title：Journal of cell science

authors： Sartorel E,Pérez-Martín J

更新日期：2012-10-01 00:00:00

abstract：:Mitosis involves considerable membrane remodelling and vesicular trafficking to generate two independent cells. Consequently, endocytosis and endocytic proteins are required for efficient mitotic progression and completion. Several endocytic proteins also participate in mitosis in an endocytosis-independent manner. He...

journal_title：Journal of cell science

authors： Ma MP,Chircop M

更新日期：2012-09-15 00:00:00

abstract：:In culture, cell confluence generates signals that commit actively growing keratinocytes to exit the cell cycle and differentiate to form a stratified epithelium. Using a comparative proteomic approach, we studied this 'confluence switch' and identified a new pathway triggered by cell confluence that regulates basemen...

journal_title：Journal of cell science

authors： Botta A,Delteil F,Mettouchi A,Vieira A,Estrach S,Négroni L,Stefani C,Lemichez E,Meneguzzi G,Gagnoux-Palacios L

更新日期：2012-09-15 00:00:00

abstract：:Perilipin family proteins (Plins) coat the surface of intracellular neutral lipid storage droplets in various cell types. Studies across diverse species demonstrate that Plins regulate lipid storage metabolism through recruitment of lipases and other regulatory proteins to lipid droplet surfaces. Mammalian genomes hav...

journal_title：Journal of cell science

authors： Hsieh K,Lee YK,Londos C,Raaka BM,Dalen KT,Kimmel AR

更新日期：2012-09-01 00:00:00

abstract：:Assembly of contractile apparatuses in striated muscle requires precisely regulated reorganization of the actin cytoskeletal proteins into sarcomeric organization. Regulation of actin filament dynamics is one of the essential processes of myofibril assembly, but the mechanism of actin regulation in striated muscle is ...

journal_title：Journal of cell science

authors： Nomura K,Ono K,Ono S

更新日期：2012-09-01 00:00:00

abstract：:Multinucleated muscle fibres arise by fusion of precursor cells called myoblasts. We previously showed that CKIP-1 ectopic expression in C2C12 myoblasts increased cell fusion. In this work, we report that CKIP-1 depletion drastically impairs C2C12 myoblast fusion in vitro and in vivo during zebrafish muscle developmen...

journal_title：Journal of cell science

authors： Baas D,Caussanel-Boude S,Guiraud A,Calhabeu F,Delaune E,Pilot F,Chopin E,Machuca-Gayet I,Vernay A,Bertrand S,Rual JF,Jurdic P,Hill DE,Vidal M,Schaeffer L,Goillot E

更新日期：2012-08-15 00:00:00

abstract：:Successful cytokinesis requires proper assembly of the contractile actomyosin ring, its stable positioning on the cell surface and proper constriction. Over the years, many of the key molecular components and regulators of the assembly and positioning of the actomyosin ring have been elucidated. Here we show that cell...

journal_title：Journal of cell science

authors： Mishra M,Huang Y,Srivastava P,Srinivasan R,Sevugan M,Shlomovitz R,Gov N,Rao M,Balasubramanian M

更新日期：2012-08-15 00:00:00

abstract：:MicroRNAs are short non-coding RNAs involved in post-transcriptional regulation of multiple messenger RNA targets. The miR-1/miR-206 family is expressed during skeletal muscle differentiation and is an integral component of myogenesis. To better understand miR-1/miR-206 function during myoblast differentiation we iden...

journal_title：Journal of cell science

authors： Goljanek-Whysall K,Pais H,Rathjen T,Sweetman D,Dalmay T,Münsterberg A

更新日期：2012-08-01 00:00:00

abstract：:Many of the cilia- and flagella-specific integral membrane proteins identified to date function to sense the extracellular milieu, and there is considerable interest in defining pathways for targeting such proteins to these sensory organelles. The flagellar glucose transporter of Leishmania mexicana, LmxGT1, is target...

journal_title：Journal of cell science

authors： Tran KD,Rodriguez-Contreras D,Shinde U,Landfear SM

更新日期：2012-07-15 00:00:00

abstract：:For all eukaryotic cilia the basal bodies provide a template for the assembly of the doublet microtubules, and intraflagellar transport provides a mechanism for transport of axonemal components into the growing cilium. What is not known is how the central pair of microtubules is nucleated or how their associated polyp...

journal_title：Journal of cell science

authors： Dymek EE,Smith EF

更新日期：2012-07-15 00:00:00

abstract：:Concomitant expression of mutant p53 and oncogenic Ras, leading to cellular transformation, is well documented. However, the mechanisms by which the various mutant p53 categories cooperate with Ras remain largely obscure. From this study we suggest that different mutant p53 categories cooperate with H-Ras in different...

journal_title：Journal of cell science

authors： Solomon H,Buganim Y,Kogan-Sakin I,Pomeraniec L,Assia Y,Madar S,Goldstein I,Brosh R,Kalo E,Beatus T,Goldfinger N,Rotter V

更新日期：2012-07-01 00:00:00

abstract：:3-Phosphoinositide-dependent protein kinase-1 (PDK1) and phospholipase C (PLC)γ1 are two key enzymes in signal transduction that control several intracellular processes. Despite the fact that PLCγ1 has been investigated for several years, the mechanisms of activation of this enzyme are still not completely clear. Simi...

journal_title：Journal of cell science

authors： Raimondi C,Chikh A,Wheeler AP,Maffucci T,Falasca M

更新日期：2012-07-01 00:00:00

abstract：:Cell fusion plays a well-recognized, physiological role during development. Bone-marrow-derived hematopoietic cells have been shown to fuse with non-hematopoietic cells in a wide variety of tissues. Some organs appear to resolve the changes in ploidy status, generating functional and mitotically-competent events. Howe...

journal_title：Journal of cell science

authors： Skinner AM,Grompe M,Kurre P

更新日期：2012-06-15 00:00:00

abstract：:Desmosomes are intercellular junctions specialised for strong adhesion that are prominent in the epidermis and heart muscle. Defective desmosomal function due to inherited mutations in the constitutive desmosomal gene desmoplakin (DSP) causes skin or heart disorders and in some instances both. Different mutations have...

journal_title：Journal of cell science

authors： Cabral RM,Tattersall D,Patel V,McPhail GD,Hatzimasoura E,Abrams DJ,South AP,Kelsell DP

更新日期：2012-06-15 00:00:00

abstract：:The protozoan parasite Giardia intestinalis (also known as Giardia lamblia) is a major waterborne pathogen. During its life cycle, Giardia alternates between the actively growing trophozoite, which has two diploid nuclei with low levels of allelic heterozygosity, and the infectious cyst, which has four nuclei and a to...

journal_title：Journal of cell science

authors： Carpenter ML,Assaf ZJ,Gourguechon S,Cande WZ

更新日期：2012-05-15 00:00:00

abstract：:Serotonin receptors 5-HT(1A) and 5-HT(7) are highly coexpressed in brain regions implicated in depression. However, their functional interaction has not been established. In the present study we show that 5-HT(1A) and 5-HT(7) receptors form heterodimers both in vitro and in vivo. Foerster resonance energy transfer-bas...

journal_title：Journal of cell science

authors： Renner U,Zeug A,Woehler A,Niebert M,Dityatev A,Dityateva G,Gorinski N,Guseva D,Abdel-Galil D,Fröhlich M,Döring F,Wischmeyer E,Richter DW,Neher E,Ponimaskin EG

更新日期：2012-05-15 00:00:00

abstract：:Podosomes are actin-rich adhesion and invasion structures. Especially in macrophages, podosomes exist in two subpopulations, large precursors at the cell periphery and smaller podosomes (successors) in the cell interior. To date, the mechanisms that differentially regulate these subpopulations are largely unknown. Her...

journal_title：Journal of cell science

authors： Bhuwania R,Cornfine S,Fang Z,Krüger M,Luna EJ,Linder S

更新日期：2012-05-01 00:00:00

abstract：:Recent evidence suggests that organization of the extracellular matrix (ECM) into aligned fibrils or fibril-like ECM topographies promotes rapid migration in fibroblasts. However, the mechanisms of cell migration that are altered by these changes in micro-environmental topography remain unknown. Here, using 1D fibrill...

journal_title：Journal of cell science

authors： Doyle AD,Kutys ML,Conti MA,Matsumoto K,Adelstein RS,Yamada KM

更新日期：2012-05-01 00:00:00